wnybubba from Bflo/ROC on 3/26/2021 10:18:41 AM:
Wow - this is really getting confusing! Seems to me that previously the Empire Service offered roll-on bike service. But, the search I just did shows that it is the Lake Shore service that now provides bike service. (I'll post that previous comment below). Meanwhile, not that long ago, I had a couple calls with AMTRAK and was assured that any station that had attendants could handle roll-on service! Granted that would mean that you still would have to have made a "rack" reservation, but loading/unloading stations are quite numerous. Also, for the record, if you bag your bike, any train can then accommodate it -- but you have tools and be willing to take it apart, etc.
Yankee John provides some current info above, but for reference,
Here is the post from September 2020:
Yankee John from Braintree, VT on 09/06/2020 06:46 PM:
There is currently no bike service on the Empire Service trains, but there is bike service on the Lake Shore Limited that runs once/day to Chicago. I just rode the Lake Shore Limited #48/49 from Schenectady to Rochester with my bike. The train was on time, an Amtrak staff person put loaded my bike on the baggage car without difficulty and returned it to me in Rochester undamaged. The only hard part is that the short distance Coach seats are at the front of the train, and the baggage car is at the back, so it's a long walk with your panniers after you drop off your bike. You need to have a reservation for the bike because the baggage car holds only hold 6 bikes. It costs $20 for the bike. The train arrived in Rochester at 2315, and it was an easy ride to the downtown hotels on well lit streets.
The Lake Shore Limited does not stop at the Exchange station in downtown Buffalo, only at the Depew station, and it arrives after midnight. The hotels hear Depew are all dull big box airport hotels. The Lakeshore used to skip Rome, and was 30 minutes faster, but apparently the tracks on the direct route the omits Rome are bad so all of the trains go through Rome even if they don't stop there. There are rumors that the tracks repairs will be completed by next year so the trip will be shorter and the train will arrive earlier. It's not expensive to reserve a taxi van in advance that can transport you to a hotel from Depew, but it will only hold 2 bikes. There is not a continuous trail along the water from downtown Buffalo to the trails leaving Buffalo north to the Erie Canal, so if you stay in a downtown hotel you will have to ride on city streets at the start of your trip. Buffalo has some great architecture, so the ride is actually quite pleasant if it's not rush hour.